LOCUS       pLV-[hsa-miR-595]        9150 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7071..7931
                     /label=Amp(R)
     promoter        8132..8672
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5676..5910
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6485..6940)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5982..6112
                     /label=SV40 pA
     misc_feature    6113..6326
                     /label=SV40 ori
     misc_feature    4997..5593
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3615
                     /label=intron
     CDS             3662..4987
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3405
                     /label=hsa-mir-595 precursor
BASE COUNT     2148 a      2372 c      2492 g      2138 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg gtgtggtgga 
     3121 gcctggccgt ccctgagcca tcaagatggt ctgcgcgtca gagtgcagca cgggccacat 
     3181 tctctctgcc atgtgcagag ggctcctgca cggaagcctg cacgcattta acaccagcac 
     3241 gctcaatgta gtcttgtaag gaacaggttg aagtgtgccg tggtgtgtct ggaggaagcg 
     3301 cctgttgctg gtttttgttg ttttgttatg tattttttgc catgttccat gctgttcctc 
     3361 taatcacatc atgtcccggg tttttgaagt ttagtgacgt ttttggctag ctcgagcttt 
     3421 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact 
     3481 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt 
     3541 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt 
     3601 tttcttccat ttcaggtgtc gtgaaaacta cccctctaga gtcgagctac cggtcgccac 
     3661 catggtgagc aagggcgagg aggataacat ggccatcatc aaggagttca tgcgcttcaa 
     3721 ggtgcacatg gagggctccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg 
     3781 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc 
     3841 cttcgcctgg gacatcctgt cccctcagtt catgtacggc tccaaggcct acgtgaagca 
     3901 ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca agtgggagcg 
     3961 cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct ccctgcagga 
     4021 cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg acggccccgt 
     4081 aatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc ccgaggacgg 
     4141 cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc 
     4201 tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt 
     4261 caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga 
     4321 acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg acccaccggt 
     4381 cgccaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag 
     4441 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga 
     4501 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg 
     4561 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac 
     4621 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt 
     4681 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc 
     4741 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg 
     4801 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc 
     4861 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt 
     4921 caccgccgac gtcgagtgcc cgaaggaccg cgcgacctgg tgcatgaccc gcaagcccgg 
     4981 tgcctgagcg gccgcgtcga caatcaacct ctggattaca aaatttgtga aagattgact 
     5041 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg 
     5101 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg 
     5161 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg 
     5221 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg 
     5281 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc 
     5341 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg 
     5401 acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc 
     5461 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct 
     5521 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc 
     5581 gcctccccgc ctggaattcg agctcggtac ctttaagacc aatgacttac aaggcagctg 
     5641 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 
     5701 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 
     5761 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 
     5821 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 
     5881 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 
     5941 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 
     6001 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 
     6061 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 
     6121 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     6181 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 
     6241 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc 
     6301 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa 
     6361 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
     6421 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
     6481 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 
     6541 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 
     6601 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 
     6661 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt 
     6721 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 
     6781 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat 
     6841 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 
     6901 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa 
     6961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca 
     7021 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc 
     7081 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc 
     7141 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt 
     7201 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt 
     7261 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg 
     7321 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact 
     7381 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg 
     7441 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga 
     7501 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg 
     7561 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa 
     7621 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac 
     7681 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc 
     7741 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca 
     7801 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga 
     7861 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta 
     7921 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc 
     7981 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc 
     8041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 
     8101 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 
     8161 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 
     8221 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 
     8281 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 
     8341 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 
     8401 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 
     8461 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 
     8521 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 
     8581 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 
     8641 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 
     8701 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 
     8761 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 
     8821 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 
     8881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 
     8941 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt 
     9001 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg 
     9061 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc 
     9121 ctcactaaag ggaacaaaag ctggagctgc 
//
